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Enzyme Practicals x2: Effect of pH

Enzyme Practicals x2: Effect of pH

Subject: Biology

Age range: 14-16

Resource type: Worksheet/Activity

Last updated

3 November 2026

zip, 828.9 KB
zip, 828.9 KB
Enzyme Practical: Effect of pH

Biology | Enzyme Activity | Ages 14–16

Investigate the effect of pH on enzyme activity using two practical options: catalase and amylase. Students test enzyme activity across pH 3–8 to explore optimum pH and how changes in pH affect reaction rates.

What’s Included?

For each enzyme:

  • Full investigation (4 pages): aim, background, hypothesis, variables, equipment, method, safety, results, calculations, graphing, conclusion and evaluation.
  • Lite practical sheet (1 page): a simplified hands-on investigation with method, safety and results table.
  • Technician notes (3 pages): equipment checklist, solution recipes, preparation, expected results, troubleshooting, safety and disposal.

The catalase investigation uses potato discs in buffered hydrogen peroxide at room temperature. The amylase investigation uses the starch–iodine method at 35 °C.

Technician notes include a citrate–phosphate buffer recipe covering pH 3–8, or the option of purchased buffer tablets. A simplified junior option using dilute acid and alkali is also included.

Preparation quantities are designed for 30 students working in pairs, with guidance for adapting other class sizes.

Files Included
  • 6 editable Word documents.
  • Print-ready PDFs.
  • Colour and black-and-white versions.

Suitable for Year 10–11 Biology (ages 14–16). Choose the full investigation for a detailed practical write-up or the Lite sheet for a shorter practical lesson.

A matching Effect of Temperature practical set (four enzyme options) is available separately.

Get this resource as part of a bundle and save up to 22%

A bundle is a package of resources grouped together to teach a particular topic, or a series of lessons, in one place.

Bundle

IGCSE Biology 0610: Enzymes Resource Bundle

IGCSE Biology 0610: Enzymes Resource **Topic 5 | Core & Extended | 2026–2028 Syllabus** Teach, investigate and revise Enzymes with this comprehensive collection of seven resources for Cambridge IGCSE Biology 0610. Combining an editable PowerPoint, structured student workbook, practical investigations, a cloze reading worksheet and examination-style questions, this collection supports learning throughout the topic. ## What's Included - **Enzymes PowerPoint:** Teaching presentation to introduce and explain key concepts. - **Enzymes Workbook:** Structured student activities, Core and Extended content, revision tasks and examination practice. - **Amylase and Agar Experiment:** Investigate the effect of temperature on enzyme activity using starch agar. - **Effect of Temperature Practical Set:** Four enzyme investigation options, with full student investigations, Lite sheets and technician notes. - **Effect of pH Practical Set:** Catalase and amylase investigations exploring the effect of pH on enzyme activity. - **Enzymes Cloze Reading Worksheet:** Five short passages with word banks to reinforce key terminology and understanding. - **Enzymes Examination Practice Questions:** Topic-based questions to consolidate knowledge and develop examination technique. ## Key Features - Designed for Cambridge IGCSE Biology 0610 (2026–2028 syllabus). - Core and Extended content. - A combination of teaching materials, student activities and practical investigations. - Technician preparation notes to support practical lesson planning. - Editable Word documents and PowerPoint, alongside print-ready PDFs in the formats supplied. - Suitable for classroom teaching, homework, revision and independent learning. ## Suitable For Cambridge IGCSE Biology 0610, Topic 5: Enzymes. Use the complete collection to teach the topic, develop practical skills, reinforce scientific vocabulary and prepare students for Cambridge examinations. *© The Science Resource Cabinet 2026. Independently produced resource; not affiliated with or endorsed by Cambridge International Education.*

£14.00

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