This pack covers Edexcel specification point 4.7: how genetic analysis has led to the suggestion of the three domains rather than the five kingdoms classification method.

The Topic Mastery Sheet shows how organisms are sorted into smaller and smaller groups, explains species, genus and binomial names, sets out the five kingdoms by the features of their cells, and explains how comparing DNA and RNA base sequences revealed the archaea and led to the three domains, with an original three-domain diagram. An exam tip comes from the June 2025 Edexcel exam. A key vocabulary table gives the exact Edexcel terms, and six common errors are corrected. The Grade 8-9 stretch section explains why appearance can mislead classification.

The Student Exam Practice Sheet (16 marks) has six questions. They progress from a multiple choice item on the domain of mushrooms and two differences between prokaryotic and eukaryotic cells through naming kingdoms from cell descriptions, which animals are most closely related from their scientific names and why horses and donkeys are separate species, to a 4-mark question on deciding the domain of a newly discovered organism, ending with a 3-mark question on base-sequence similarity data. Answers follow Edexcel mark scheme conventions; the prokaryote and eukaryote differences follow the official June 2025 Edexcel Combined Science 1BH mark scheme.

Project the mastery sheet for a ready-made revision lesson, set the practice sheet as homework or a timed check, or use the pack for intervention and independent study. PDF and editable Word versions are both included. Written for Edexcel GCSE Combined Science (1SC0); points that appear only in the separate GCSE Biology specification are not included.

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