Amylase Practical – Effect of Temperature

Cambridge IGCSE Biology 0610 | Enzymes

Investigate how temperature affects enzyme activity with this ready-to-use practical on the action of amylase on starch. Students follow a starch-agar method, compare results at different temperatures and use their observations to explore the effect of temperature on enzyme activity.

What’s Included

• Student worksheet (4 pages): Practical instructions, results recording and questions to help students interpret their findings.
• Lite worksheet (1 page): A shorter version for revision or more focused practical work.
• Technician preparation sheet (2 pages): Equipment checklist, preparation instructions and solution recipes, with quantities for 30 students working in pairs.
• Three temperatures: Compare amylase activity at 20°C, 37°C and 80°C.
• Results analysis: Flood the starch agar with iodine and measure the clear zones to compare enzyme activity.
• Colour and black-and-white editions: Editable Word documents and print-ready PDFs.

Key Features

• Practical investigation of temperature and enzyme activity.
• Clear instructions for students and separate preparation guidance for technicians.
• Includes safety and preparation notes, plus guidance for adapting quantities to different class sizes.
• Designed for straightforward classroom use.

Suitable For

Cambridge IGCSE Biology 0610 Topic 5: Enzymes, as well as equivalent Biology courses covering enzyme activity, temperature and denaturation.

Ideal for practical lessons, investigation skills and examination revision.

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